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How to measure the true feed protein in the bacterial protein?
 <SPAN title=实验室常用考马斯亮蓝G-250法,不是以测定 style="BACKGROUND-COLOR: #fff" 氮?含量为基础。?>Commonly used laboratory Coomassie Brilliant Blue G-250 method, not to determine "nitrogen" content-based. Bradford is a methyl-substituted triphenyl methane sulfonic acid molecule in the blue dye, with maximum absorption at 465nm value. Coomassie Brilliant Blue G-250 with protein interactions through the formation of the protein van der - Coomassie blue complex solution, the dye caused by the location of the maximum red-shifted absorption λmax at 595nm at the maximum absorption value. As the protein - Coomassie brilliant blue complex absorbance at 595nm is much higher than at the Coomassie brilliant blue at the light at the 465nm absorption, therefore, can greatly improve the sensitivity of protein determination. Protein - complex solution of Coomassie brilliant blue color shades and protein concentration. Color differences in Solution colorimetric determination for quantitative analysis of proteins, forming reaction color stability, high sensitivity, low protein quality 1ug about the test. However, molecules with Coomassie Brilliant Blue G250 benzene ring.
You can also use the "UV absorption method", which is not based on determination of "nitrogen" content-based, which the absorbance at 280nm is the most commonly used UV absorption method. The principle is that protein tyrosine, phenylalanine and tryptophan residues of the benzene ring containing conjugated double bonds, so that the nature of the protein with the absorption of UV light. Absorption peak at 280nm at its absorbance (ie optical density) and proportional to protein content. In addition, the protein solution at 238nm and the light absorption is proportional to peptide concentration, the use of a certain wavelength, the absorbance of protein solution with the positive relationship between protein concentration, protein content can be the determination of melamine and other compounds containing conjugated double bonds the benzene ring, but no peptide bond, so the 238nm absorbance value can be ruled out at the other interfere with the benzene ring conjugated double bonds.
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